Browsing Fakultät für Biologie und Psychologie (inkl. GAUSS) by Advisor "Lührmann, Reinhard Prof. Dr."
Now showing items 1-11 of 11
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Investigation of the structure of spliceosomal complexes from the yeast S. cerevisiae
(2021-03-19)Nuclear pre-mRNA splicing is catalysed by the spliceosome, a multi-megadalton ribonucleoprotein (RNP) complex. It assembles de novo for each round of splicing on premRNA intron with stepwise binding of five small nuclear ... -
Identification and characterization of small molecule inhibitors of pre-mRNA splicing that block spliceosome assembly at novel stages
(2016-02-19)The spliceosome is a highly dynamic, megadalton ribonucleoprotein (RNP) molecular machine that catalyses the important step of the post-transcriptional processing of eukaryotic precursor messenger RNA (pre-mRNA), called ... -
Biochemical and structural characterization of spliceosomes purified at defined stages of assembly from the yeast S. cerevisiae
(2013-09-06)Splicing of pre messenger RNA (pre mRNA) is catalyzed by the spliceosome, a multimegadalton ribonucleoprotein (RNP) comprising several small nuclear (sn) RNPs and numerous proteins. The spliceosome assembles on its pre ... -
Untersuchungen zum Mechanismus der katalytischen Aktivierung von Spleißosomen aus Saccharomyces Cerevisiae
(2013-02-13)Pre-mRNA splicing is catalyzed by the spliceosome, a multimegadalton ribonucleoprotein (RNP) complex. It assembles anew on each pre-mRNA intron by the stepwise binding of five snRNPs (U1, U2, U4, U5 and U6) and numerous ... -
The Molecular Architecture and Structure of the Human Prp19/CDC5L Complex and 35S U5 snRNP
(2011-09-22)Pre-mRNA splicing, the two consecutive transesterification reactions leading to intron removal and exon ligation, is catalyzed by the spliceosome, a highly dynamic, multi-megadalton molecular machinery. The spliceosome is ... -
Electron microscopic localization of tagged proteins in the yeast S. cerevisiae spliceosomal U4/U6.U5 trisnRNP
(2008-10-30)Pre-mRNA splicing is catalyzed by a macromolecular machine called spliceosome . The spliceosome is assembled in a stepwise manner from the small nuclear ribonucleoprotein particles (snRNPs), ... -
Characterization of cytoplasmic bodies involved in 5' to 3' mRNA degradation in human cells
(2008-04-23)In the context of gene expression regulation, discontinuing translation of mRNAs occurs by evolutionarily conserved mechanisms whose executors have been described to be associated with various types of cytoplasmic ... -
Determination of the Structure of the Spliceosomal U6 snRNP from Yeast, <i>Saccharomyces cerevisiae</i>
(2007-08-09)The U6 small nuclear RNA (snRNA) undergoes major conformational changes during the assembly of the spliceosome and catalysis of splicing. It associates with the specific protein Prp24p, ... -
Isolation and Characterization of Human Precatalytic Spliceosomal B Complexes
(2007-04-26)The spliceosomal B complex is the substrate that undergoes catalytic activation leading to catalysis of pre-mRNA splicing. Previous characterization of this complex was performed in the ... -
Investigation of the higher order structure of the spliceosomal RNA network
(2007-02-05)Majority of the genes of higher eukaryotes possess introns (internal non-coding sequences) which have to be excised via splicing to generate translatable mRNAs. Recognition and pairing of ... -
Biochemische und zellbiologische Untersuchungen zur Rolle der Cajal Bodies bei der Zusammenlagerung spleißosomaler UsnRNP Partikel
(2005-06-27)Eukaryotic pre-mRNA splicing is catalysed by a large macromolecular complex called the spliceosome. One central unit of the spliceosome is the [U4/U6.U5] tri-snRNP particle that consists ...