Browsing Fakultät für Biologie und Psychologie (inkl. GAUSS) by Referee "Tittmann, Kai Prof. Dr."
Now showing items 1-20 of 25
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Characterization of the Phosphomimetic Mutant Y39E of a-Synuclein
(2024-08-06)α-Synuclein (aSyn) has long been identified as a key factor in the pathogenesis of Parkinson's Disease (PD), with familial mutations in the SNCA gene contributing to the aberrant aggregation of aSyn. Its aggregation results ... -
Macromolecules in Motion by High-resolution Cryo-EM
(2024-05-06)Protein synthesis, the translation of the genetic code into a defined sequence of amino acids, is a central, highly dynamic and regulated step in gene expression. The well-defined bacterial translation system enables to ... -
Improving electron microscopic hardware to push the resolution limits in single particle cryo-EM
(2022-07-19)Electron cryomicroscopy (cryo-EM) is a powerful tool in structural biology for determining 3D structures of macromolecular complexes. In the previous decade, many technological advancements have been introduced to improve ... -
Manipulating the Conformational Landscape of Large Macromolecular Complexes
(2022-04-08)The de novo synthesis of fatty acids is one of the most central metabolic pathways in all forms of life. Among all known organisms, fatty acids are highly essential. Besides their role as energy resource, they are also ... -
Structural characterization of human spliceosome activation by cryo-EM
(2022-01-27)Eukaryotic genes are transcribed as precursor mRNA (pre-mRNA), in which coding regions (exons) are interrupted by non-coding regions (introns). Introns are excised and exons are ligated together in a two-step process ... -
Reconstitution of the Integrator complex and its interaction with paused transcription elongation complex of Pol II-DSIF-NELF
(2021-10-19)The Integrator complex (INT) is the latest and largest multi-subunit protein complex to be added to the list of factors involved in RNA Polymerase II (Pol II) transcription. INT consist of 15 subunits with an estimated ... -
One-step RESOLFT with a positively switchable RSFP with improved deactivation kinetics
(2020-10-01)Interaction and spatial organization of subcellular structures within living cells are an essential aspect of biological research, but conventional microscopy can elucidate these properties only to a finite extent as it is ... -
Structural and biochemical characterization of c-di-AMP synthesizing enzymes
(2020-06-11)One major concern of today’s life is the increase of antimicrobial resistance and the rising num-ber of multi drug resistant bacterial species. There is an urgent need of identifying new antibi-otic drug targets since ... -
Specific ubiquitin-dependent protein degradation requires a trimeric CandA complex in Aspergillus nidulans
(2019-05-20)E3 cullin-RING ubiquitin ligase complexes are activated by modification of their cullin scaffold protein with the ubiquitin-like modifier Nedd8. Deneddylases as the COP9 signalosome (CSN) or Den1/A interact and destabilize ... -
Cytoskeletal reorganization in human blood platelets during spreading
(2017-06-21)Anucleate, human blood platelets play an important role in haemostasis to prevent excessive blood loss from a vascular damage. Their actin and microtubule (MT) cytoskeleton is especially responsible for bringing about ... -
Modulating the aggregation of alpha-synuclein and prion protein with small molecules.
(2016-11-07)As living and health conditions improve in modern societies, the percentage of older people rises. A byproduct of this ageing is the marked increase of cases of cancer, cardiovascular, and neurodegenerative diseases. Many ... -
Phosphorylation dependent stability control of the deneddylase DenA and its impact on Aspergillus nidulans development
(2016-04-05)Summary Malfunctioning protein degradation in higher eukaryotes is associated with numerous diseases, including neurodegenerative disorders and cancer. Understanding regulation mechanisms of protein degradation is thus ... -
Deciphering the Catalytic Mechanism of the Zn Enzyme Glutaminyl Cyclase and the Deduction of Transition-State Analog Inhibitors
(2015-07-20)Zn(II)-dependent glutaminyl cyclase (QC) converts N-terminal glutamine or glutamate residues of peptides and proteins. The reaction product is in both cases N-terminal pyroglutamic acid (pyroGlu). In animals the glutaminyl ... -
Characterization of chondrogenic progenitor cells using mass spectrometry and multidimensional separation approaches
(2015-07-03)Modern day mass spectrometry (MS) has evolved into a powerful analytical technique, central to the field of proteomics. Currently, the identification and quantification of proteins from different biological samples can be ... -
Investigations of sirtuin metabolism
(2015-04-15)Sirtuins are a class of histone deacetylases (HDACs) found in all organisms in all three domains of life. Unlike other classes, which hydrolyze water, sirtuins require NAD+ as co-substrate and form nicotinamide and the ... -
Molecular Dynamics Studies of the Phi29 Connector-DNA complex
(2015-04-10)During replication of the Phi29 bacteriophage, a DNA packaging motor packages the viral DNA into the procapsid against a maximum pressure difference of ~60 atm, which is generated by the already packed DNA. Several models ... -
A method for the genetically encoded incorporation of FRET pairs into proteins
(2014-09-03)Proteins are composed of 20 canonical amino acids whose unique arrangements predefine a protein’s structure and function. Importantly, most proteins are not static conformations but rather very dynamic entities that undergo ... -
Protein interactions along the presequence import pathway
(2014-05-09)In this thesis the mechanisms of protein transport by the translocase of the inner mitochondrial membrane (TIM23) was investigated. Purified presequence peptides containing a p-benzophenyalalanine were established as a ... -
Structural characterization of the minimal human RISC-loading complex
(2014-03-07)Gene expression is the prerequisite for protein biosynthesis in all cells. Rapid and fine-tuned control of gene expression in response to environmental changes is of great importance. A major gene regulation mechanism in ... -
Regulation der Aktivität und Lokalisation von Antiterminatorproteinen der BglG-Familie
(2012-11-12)The regulation of gene expression is essential for all bacteria and allows adapting to changing life conditions. Different transcription and translation factors have an important impact on gene regulation. The antermination ...