Browsing Fakultät für Physik (inkl. GAUSS) by Referee "Köster, Sarah Prof. Dr."
Now showing items 1-20 of 28
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Investigation of Cells and Cellular Components with X-Rays in Liquid Sample Environments
(2024-08-07)Due to their short wavelength, X-rays offer a high spatial resolution and a high ability to penetrate soft condensed matter. Therefore, X-rays are well-suited for investigating structures and structural changes in in-vitro ... -
Interactions Within and Between Cytoskeletal Filaments
(2023-05-26)Cellular mechanics are determined by the cytoskeleton which consists of three different kind of biopolymers – actin filaments, microtubules and intermediate filaments – which form an intricate network. Each filament type ... -
New Approaches to Cryo-EM Image Processing
(2022-12-16)Using cryo-electron microscopy (cryo-EM), it is possible to resolve structures of biological macromolecules by averaging numerous projections of macromolecules (particle images) frozen in vitrified ice and imaged using ... -
Low-dose, high-throughput scanning small-angle X-ray scattering of adherent mouse embryonic fibroblasts
(2022-02-24)Biological cells are highly variable: differences in e. g. gene expression emerge even among monoclonal cells in the same culture dish. The characterization of a highly variable statistical population calls for high-throughput ... -
Localizing and tracking of single molecules with a MINFLUX-microscope for various applications
(2022-01-25)Recently MINFLUX microscopy was first published, a technique that localizes single fluorescent molecules by probing with a structured illumination excitation beam featuring a central intensity minimum. Single nanometer ... -
Multi-parameter assessment of mechano-sensitivity driven differentiation of human mesenchymal stem cells
(2020-12-22)What factors and conditions control stem cell differentiation is currently one of the major questions in biomedical research. What factors ultimately influence the cells’ decision is unclear but it has been shown, that ... -
Adaptive Scanning for STED Microscopy
(2020-02-06)Optical nanoscopy allows for highly specific imaging of biological tissue, cellular components and even single molecules and has therefore become an integral part of modern biomedical research. In STED microscopy, being ... -
isoSTED microscopy for live cell imaging
(2019-05-08)Far-field fluorescence microscopy is a versatile tool for the non-invasive investigation of intracellular structures and thus for live cell imaging. This is a major advantage over other microscopy methods such as electron ... -
Mechanosensing and Symmetry of Potassium Channels Studied by Molecular Dynamics Simulations
(2018-10-02)Potassium channels are relevant in a variety of physiological processes in all three kingdoms of life. In mammals these processes including nerve signaling, heartbeat regulation, and osmotic regulation of cells. Thus a ... -
Time-resolved X-ray phase-contrast tomography
(2018-06-27)X-ray tomography allows to obtain the three-dimensional (3d) structure of weakly interacting objects, such as tissue samples in biomedical imaging or nanoscale structures in materials science, with high spatial resolution. ... -
Localizing and tracking of fluorescent molecules with minimal photon fluxes
(2018-01-19)In this thesis MINFLUX is presented, a novel optical scheme to determine the position of individual photon emitters in space. By probing the emitter with adapted illumination profiles featuring an intensity zero, the number ... -
Advanced x-ray multilayer waveguide optics
(2017-09-12)The aim of this thesis is to demonstrate that x-ray waveguide optics can be gen- eralized from a single guiding film to an array of planar waveguides, enabling more complex and controllable phenomena of field propagation ... -
Tomographic STED Microscopy
(2017-03-09)Due to its non-invasive access to sub-cellular structures, far-field fluorescence microscopy constitutes one of the most prevalent methods in the life sciences. For more than one century, it had been common knowledge ... -
Single-Molecule Metal-Induced Energy Transfer: From Basics to Applications
(2016-12-16)Single-molecule detection and spectroscopy have revolutionized the field of fluorescence microscopy. Due to their enormous potential in studying physics, chemistry and biology at molecular level, the number of single-molecule ... -
Liquid Crystal Microswimmers - from single entities to collective dynamics
(2016-11-15)Recently, artificial self-propelled systems at low Reynolds numbers have gained a lot of attention. The main interest lies in mimicking well-studied systems, e.g. the active Brownian motion of bacteria, or in gaining deeper ... -
Elasticity of basic structural element in anisotropic macromolecular networks
(2016-09-27)Semiflexible filaments are abundant in biological materials. From cells to the extracellular environment, these kind of filaments are found. They are important for many biological organizations and processes. The maintenance ... -
Investigating cellular nanoscale with x-rays from proteins to networks
(2016-08-12)In this work, two macromolecules, both essential components of cells (keratin protein and DNA nucleic acid) were studied by X-ray techniques. I described the implementation and use of different methods to investigate these ... -
Scanning X-Ray Nanodiffraction on Dictyostelium discoideum
(2015-11-06)In the recent years, novel focussing optics for synchrotron radiation sources became available, which now allow focussing the x-radiation down to 100\,nm (FWHM). Thus, the typically poor spatial resolution of Small Angle ... -
DNA-based molecular force sensors in cytoskeletal networks and cells
(2015-07-30)Eukaryotic cells are highly active and undergo force generating processes such as cell adhesion, migration and division. The cytoskeleton, a polymer meshwork in cells, plays an important role in these cellular processes. ... -
Mechanics of Intermediate Filaments
(2014-07-02)The eukariotic cytoskeleton consists of three major filament types: actin filaments, microtubules and intermediate filaments. While the first two filament types are highly conserved, intermediate filaments vary between ...