Dokumente Fakultät für Biologie und Psychologie (inkl. GAUSS) nach Gutachter "Ficner, Ralf Prof. Dr."
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Etablierung und Analyse von 'knock-out' Mausmodellen der σ1 Untereinheiten des AP 1 Komplexes
(2009-02-10)The multimeric adaptor protein complex 1 (AP-1) is essential for clathrin coated vesicle assembley mediating protein sorting between the trans-Golgi network (TGN) and the endosomal compartment. ... -
Expression analysis of Drosophila melanogaster microRNAs
(2007-01-31)miRNAs are tiny regulatory RNAs of 20-25 nucleotides (nt) in length that are endogenously expressed in cells. Hundreds of miRNA genes have been identified in plants and animals. Together they form a large family of regulatory ... -
Expression, Charakterisierung und Kristallisation der spleißosomalen Proteine SMNrp, U4/U6-60k und U4/U6-90k
(2006-01-20)The U4/U6-20k/60k/90k-complex, a component of the U4/U6*U5-snRNP-complex, and SMNrp are involved in the assembly of the human spliceosome. It was suggested that the recruiting of the ... -
Free energy calculations of protein-ligand complexes with computational molecular dynamics
(2008-11-07)The accurate calculation of binding free energies for protein-ligand complexes is a field of extensive research. The accuracy in such free energy calculations from atomic-level molecular ... -
Function of Elongation Factor P in Translation
(2016-04-25)The ribosome translates the genetic information encoded in the mRNA into amino acid sequence of a peptide chain. The catalytic site of the ribosome where peptide bonds are made, the peptidyl transferase center, consists ... -
Functional and structural investigation of spliceosomal snRNPs
(2009-01-20)The expression of most eukaryotic protein-encoding genes involves precursor messenger RNA (pre-mRNA) processing steps including pre-mRNA splicing. Pre-mRNA splicing is catalyzed by a ... -
Functional gene analysis in cultured vertebrate cells using siRNA mediated gene silencing
(2005-02-23)Gene expression can be silenced in a sequence-specific manner by use of small interfering RNAs (siRNAs) in a process that is known as RNA interference (RNAi). The application of RNAi ... -
Die Funktion von NLP1 im CRM1-abhängigen Protein-Export aus dem Zellkern
(2011-08-05)Export of most proteins from the nucleus to the cytosol is mediated by the export factor CRM1. In the presence of RanGTP CRM1 binds to a hydrophobic region within the cargo protein, the NES ... -
Funktionelle Charakterisierung bakterieller Histondeacetylase-ähnlicher Amidohydrolasen (HDAH)
(2005-12-05)Until today, there is only little information about posttranslational modifications, especially acetylation of polypeptides in procaryotes to find in the literature. This thesis tries to ... -
Eine funktionelle Charakterisierung der frühendosomalen SNARE-Proteine
(2009-06-19)Most intracellular transport processes occur via budding and fusion of vesicles, where fusion is dependent on SNARE proteins. Fusion is preceded by docking, the close contact of vesicles ... -
GlmY and GlmZ: a hierarchically acting cascade composed of two small RNAs
(2014-09-10)In Escherichia coli and other enterobacteria, the homologous small RNAs (sRNAs) GlmY and GlmZ act in a hierarchical manner to feedback control expression of key enzyme glucosamine-6-phosphate (GlcN6P) synthase GlmS. Enzyme ... -
Die Interferenz des Tumorsuppressor-Homologen p63 mit dem kanonischen Wnt-Signalweg
(2010-02-16)The tumor suppressor homologue p63 has an essential role in the proper development of squamous epithelia, limb buds and skin appendages, like hair follicles, teeth and glands. Another ... -
Investigation of glycolysis in Bacillus subtilis
(2010-05-19)Bacillus subtilis is a model organism for Gram-positive bacteria and object for biotechnological applications. The extensive investigation of this bacterium makes it one of the best studied ... -
Investigation of the higher order structure of the spliceosomal RNA network
(2007-02-05)Majority of the genes of higher eukaryotes possess introns (internal non-coding sequences) which have to be excised via splicing to generate translatable mRNAs. Recognition and pairing of ... -
Investigation of the mitochondrial contact site and cristae organizing system and its role in cristae formation
(2020-10-27)Mitochondria are essential organelles in eukaryotic cells that feature a unique doublemembrane architecture. The mitochondrial inner membrane forms numerous invaginations, named cristae, which can adopt different shapes ... -
Investigation of the structure of spliceosomal complexes from the yeast S. cerevisiae
(2021-03-19)Nuclear pre-mRNA splicing is catalysed by the spliceosome, a multi-megadalton ribonucleoprotein (RNP) complex. It assembles de novo for each round of splicing on premRNA intron with stepwise binding of five small nuclear ... -
Investigations into the mode of action of the DNA uridine endonuclease Mth212 of Methanothermobacter thermautotrophicus ΔH
(2010-03-05)Spontaneous hydrolytic deamination of cytosine to uracil is one of the most frequent DNA modifications during cellular everyday metabolism. To counteract this mutagenic effect, most organisms ... -
Kristallisation von GGA-Proteinen und röntgenkristallographische Untersuchungen am Cα-Formylglycin Generierenden Enzym
(2007-03-05)GGA proteins are a family of monomeric clathrin adaptors that mediate the sorting of mannose-6-phosphate receptors between the trans-Golgi network and endosomes. Crystal structures of isolated GGA protein VHS, GAT and GAE ... -
Localization and dimerization of the ABC half transporter rAbcb6 as compared to rAbcb7
(2006-07-28)Rat Abcb6 (rAbcb6) is an ABC half transporter of the ABCB subfamily and is expected to require a dimerization partner to perform its function. The main aim of this thesis was to detect a ... -
Mass Spectrometric Analyses of Post-Translationally Modified Proteins
(2011-01-05)Protein post-translational modifications (PTMs) possess key functions in the regulation of various cellular processes. In this thesis, several new technologies are developed to map protein ...