Browsing Fakultät für Biologie und Psychologie (inkl. GAUSS) by Advisor "Ficner, Ralf Prof. Dr."
Now showing items 1-20 of 24
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Structure-based Search for novel c-di-AMP Synthase Inhibiting Fragments
(2023-03-24)One of the major threats for the global health system is the increasing number of antibiotic resistant bacterial strains. The misuse of antibiotics during the Covid pandemic aggravates these problems. Therefore, the ... -
Structural and biochemical characterization of c-di-AMP synthesizing enzymes
(2020-06-11)One major concern of today’s life is the increase of antimicrobial resistance and the rising num-ber of multi drug resistant bacterial species. There is an urgent need of identifying new antibi-otic drug targets since ... -
Biochemical studies of the synaptic protein otoferlin
(2015-08-27)The C2 domain containing protein otoferlin is required for a late step in exocytosis from auditory inner hair cells and was hypothesized to act as a synaptic Ca2+ sensor. More roles for otoferlin in hair cell synaptic ... -
Structural and functional studies on GTPases involved in eukaryal translation initiation
(2015-06-05)Translation, the process of mRNA-encoded protein biosynthesis, is one of the fundamental processes that are universally conserved in extant cellular life. In all its major stages, namely initiation, elongation, termination ... -
Strukturelle und funktionale Analyse der acetylierten kleinen GTPase Ran
(2015-03-19)The nucleus is the most prominent feature of every eukaryotic cell. Since the nucleus contains virtually all the genetic information it separates the transcription and translation. This separation requires a bidirectional ... -
Structural characterization of the minimal human RISC-loading complex
(2014-03-07)Gene expression is the prerequisite for protein biosynthesis in all cells. Rapid and fine-tuned control of gene expression in response to environmental changes is of great importance. A major gene regulation mechanism in ... -
Strukturelle Untersuchungen zum Velvet-Komplex aus Aspergillus nidulans
(2013-03-22)The regulation of secondary metabolism and development in Aspergillus nidulans are two linked processes. Their regulation is dependent on light. The molecular basis for this connection is a complex composed of the two ... -
Structural and functional characterization of proteins involved in the biogenesis of spliceosomal U snRNPs
(2009-09-11)One major hallmark of eucaryotic organisms is the subdivision of their cells into different, membrane enclosed compartments. In their central compartment, the nucleus, eucaryotes store their ... -
Structural characterization of the lysosomal 66.3 kDa protein and of the DNA repair enzyme Mth0212 by means of X-ray crystallography
(2009-06-17)This PhD thesis is divided into two parts both dealing with structural characterization of macromolecules by means of X-ray crystallography. Part I concerns the lysosomal 66.3 kDa protein ... -
Kristallstrukturanalyse des spleißosomalen DEAD-Box Proteins hPrp28
(2008-06-25)DEAD-box proteins belong to the helicase superfamily. They use the energy of ATP hydrolysis to dissociate RNA-RNA or RNA-protein interactions and therefore to catalyze rearrangements in ... -
Thermodynamische und strukturelle Charakterisierung Importinβ-abhängiger Kernimportprozesse
(2008-03-18)Active nuclear transport of cytoplasmic substrates into the nucleus is mediated by transport receptors also termed importins. Among them the nuclear import receptor importinβ (Impβ) is presumably the most versatile one: ... -
Strukturelle Charakterisierung der C-terminalen Domäne des spleißosomalen DExD/H-Box Proteins hPrp22
(2008-02-26)Splicing of pre-mRNA is one of the most dynamic processes in eukaryotic cells. In addition to the U snRNAs, there is a multitude of proteins involved in the splicing cycle. Some of them are mobile factors; others are ... -
Structural Analysis of the 50S Ribosomal Stalk
(2007-06-27)Protein biosynthesis represents a dynamic process that takes place on the ribosome and is driven by translation factors. Some of these factors are GTP binding proteins. They possess a limited ... -
Struktur-Funtionsbeziehung in den spleißosomalen Proteinen des U4/U6•U5 tri-snRNP
(2007-01-29)Subject of this thesis is the splicesosmal U5-particle. The functional relationship of the proteins is investigated by means of limited proteolysis and spectroscopy. Due to the splicesosome's ... -
Versuche zur Strukturaufklärung bakterieller Thiouridin Synthetasen
(2006-06-02)The tRNA of most organisms is postranscriptionally modified. The sulfurtransferases ThiI and MnmA modify tRNAs by the incorporation of sulfur. In archaea and bacteria two different roles ... -
Expression, Charakterisierung und Kristallisation der spleißosomalen Proteine SMNrp, U4/U6-60k und U4/U6-90k
(2006-01-20)The U4/U6-20k/60k/90k-complex, a component of the U4/U6*U5-snRNP-complex, and SMNrp are involved in the assembly of the human spliceosome. It was suggested that the recruiting of the ... -
Crystal structure of a human U5 snRNP specific binary complex and crystal structure of a histone deacetylase-like bacterial amidohydrolase
(2005-12-09)The crystal structure of the complex comprising the GYF-domain of U5-52K and the U5-15K protein has been determined at 2.35 Å resolution. The protein U5-52K (Lin1p) is a component of yeast ... -
Veranschaulichung subzellulärer physikalischer Kräfte biochemischen und mechanischen Ursprungs mittels FRET
(2005-12-09)The cellular cytoskeleton is composed of microfilaments, intermediate filaments, microtubules and a host of accessory proteins that control its dynamics during different, cell-type and ... -
Präparation und röntgenkristallographische Untersuchungen an archaebakteriellen Box C/D sRNPs und einer neuartigen Glukosyltransferase aus Thermotoga maritima MSB8
(2005-10-18)A variety of posttranscriptional nucleotide modifications in ribosomal ribonucleic acids (RNAs) are directed by nucleolar RNAs (snoRNAs). The two major classes involved in these modifications ...