Browsing Naturwissenschaften, Mathematik und Informatik by Referee "Jakobs, Stefan Prof. Dr."
Now showing items 1-20 of 20
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Structural and functional characterization of TIM22 complex in the inner mitochondrial membarne
(2024-08-16)Mitochondria have gained significant attention in disease models due to their crucial role in cellular energy production and metabolic activity. Understanding how mitochondria maintain their function and interact with other ... -
NMR Spectroscopy of Biomolecular Liquid-Liquid Phase Separation
(2024-02-09)Phase separation of biomolecules has transformed our understanding of cell organization and function, motivating intensive research in the last decade. New evidence for their critical role in mechanisms essential for ... -
STED microscopy with two-photon photoactivation in the visible range & Application of generative adversarial networks for image reconstruction in STED microscopy
(2023-01-31)The resolution limit of classical optical far-field microscopes was broken with the invention of super-resolution microscopy. Today, a variety of these techniques achieving different degrees of resolution exist. Image formation ... -
Mechanisms of homologous chromosome synapsis and recombination in mammalian meiosis
(2022-10-13)Homologous chromosome synapsis and recombination are key events that occur during meiotic prophase and result in the formation of chromatin structures called chiasmata. These chromatin structures physically connect ... -
Localizing and tracking of single molecules with a MINFLUX-microscope for various applications
(2022-01-25)Recently MINFLUX microscopy was first published, a technique that localizes single fluorescent molecules by probing with a structured illumination excitation beam featuring a central intensity minimum. Single nanometer ... -
Investigation of the mitochondrial contact site and cristae organizing system and its role in cristae formation
(2020-10-27)Mitochondria are essential organelles in eukaryotic cells that feature a unique doublemembrane architecture. The mitochondrial inner membrane forms numerous invaginations, named cristae, which can adopt different shapes ... -
One-step RESOLFT with a positively switchable RSFP with improved deactivation kinetics
(2020-10-01)Interaction and spatial organization of subcellular structures within living cells are an essential aspect of biological research, but conventional microscopy can elucidate these properties only to a finite extent as it is ... -
isoSTED microscopy for live cell imaging
(2019-05-08)Far-field fluorescence microscopy is a versatile tool for the non-invasive investigation of intracellular structures and thus for live cell imaging. This is a major advantage over other microscopy methods such as electron ... -
Investigating the Role of ROMO1 in Mitochondrial Protein Import and Inner Membrane Morphology
(2018-10-18)Mitochondria are vital organelles that perform fundamental functions such as ATP synthesis and iron-sulfur cluster formation. More than 99% of the mitochondrial proteome is imported by dedicated translocation machineries. ... -
In vivo super-resolution live-cell RESOLFT-microscopy of Drosophila melanogaster and Arabidopsis thaliana
(2018-04-23)The optical resolution of conventional light microscopy is limited by the diffraction barrier of light. This resolution limit impedes the characterization of cellular structures featuring a size smaller than half the ... -
Gerichtete Evolution und Charakterisierung von Bakteriophytochromen für die tiefrote, hochauflösende Fluoreszenzmikroskopie
(2017-10-18)The green fluorescent protein (GFP) and its homologues have revolutionized molecular biology through their considerable utility as genetic probes, among myriad other applications. Moreover, in recent decades, the ... -
Etablierung eines Nachweisverfahrens zur Untersuchung der räumlichen und zeitlichen Verteilung mitochondrial translatierter Proteine mit hochauflösender STED-Mikroskopie durch metabolische Markierung mit nicht-kanonischen Aminosäuren
(2017-09-14)Metabolic labeling of nascent polypeptides using non-canonical amino acids is a valuable tool enabling researchers to introduce bioorthogonal functional groups into proteins. These can subsequentially be used for identifying ... -
Cytoskeletal reorganization in human blood platelets during spreading
(2017-06-21)Anucleate, human blood platelets play an important role in haemostasis to prevent excessive blood loss from a vascular damage. Their actin and microtubule (MT) cytoskeleton is especially responsible for bringing about ... -
Untersuchung der submitochondrialen Verteilung von Oxa1 und Mba1 in Saccharomyces cerevisiae
(2016-04-21)The mitochondria of eucaryotic cells are involved in several cellular processes and fulfill a multitude of fundamental tasks, such as the production of energy via the process of oxidative phosphorylation. They exhibit a ... -
Untersuchung der sub-mitochondrialen Proteinverteilung von MICOS mittels STED-Mikroskopie
(2015-09-28)Mitochondria are essential components of eukaryotic cells. They consist of an outer and inner membrane, which itself is divided into the inner boundary membrane and the cristae. The transition from the inner boundary ... -
Evolution and epigenetic regulation of RNA-mediated duplicated genes in Arabidopsis
(2015-06-16)Gene duplications allow for protein functional diversification and accelerate genome evolution. Occasionally, the transposon amplification machinery reverse-transcribes mRNA of a gene, integrates it into the genome and ... -
Untersuchung der Heterogenität submitochondrialer Proteinverteilungen mit hochauflösender Mikroskopie
(2015-01-15)Mitochondria are essential organelles of eukaryotic cells. They fulfill a multitude of fundamental tasks: besides their outstanding significance for energy production they possess a central role in metabolism, ion homeostasis ... -
Untersuchung der sub-mitochondrialen Lokalisation des MINOS-Komplexes in humanen Zellen
(2014-05-28)MINOS (mitochondrial inner membrane organizing structure) is a large hetero-oligomeric complex in the inner mitochondrial membrane. Originally identified in mitochondria of yeast cells, components of the complex were found ... -
Cardiac T-Tubule Membranes - Nanostructure and Remodeling Mechanisms in Disease
(2013-09-03)Transverse tubules (T-tubules, TTs) are continuous invaginations of the plasma membrane which form a complex network of excitable membranes inside mammalian ventricular cardiomyocytes. The TT network couples electrical ... -
Generation of Novel Photochromic GFPs: Fluorescent Probes for RESOLFT-type Microscopy at Low Light Intensities
(2012-05-23)The resolution of a conventional light microscope is limited by diffraction. Hence, light microscopy failed to discern objects that are closer together than half the wavelength of light. STED (STimulated Emission ...