Browsing GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften by Advisor "Steinem, Claudia Prof. Dr."
Now showing items 1-13 of 13
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Investigation of the fusion machinery reconstituted into novel pore-spanning membranes
(2024-09-06)The release of neurotransmitter into the synaptic cleft is achieved by fusion of synaptic vesicles with the presynaptic membrane. This essential process in the neuronal signal transmission is driven by the soluble ... -
Mechanistic Investigation of the Mode of Action of the Antimicrobial Peptide Lugdunin in Lipid Membranes
(2024-01-26)Dissemination of infections originating from antibiotic-resistant bacteria constitutes an escalating threat to global healthcare systems further compounded by the stagnant development of novel antibiotics. Therefore, the ... -
Giant plasma membrane vesicle-derived in vitro systems to study the structure and dynamics of CaV1.3 channel clusters
(2023-11-06)The voltage-gated calcium channel 1.3 (CaV1.3) has plentiful tasks in the human body. One of them is the excitation-contraction coupling within the heart, where it is known to form CaV1.3 channel cluster. However, their ... -
Dynamics and self-organization of membrane-bound minimal actin and actomyosin cortices based on ezrin-PtdIns[4,5]P2 linkage
(2022-11-22)The cellular cortex is a thin actin layer attached to the cytoplasmatic plasma membrane leaflet of mammalian cells. Through a complex interaction with hundreds of actin binding proteins (ABPs), controlling e.g. the cortex ... -
Droplet-based giant unilamellar vesicles as a tool for pore-spanning membrane preparation and transmembrane protein reconstitution
(2022-11-22)Model membranes represent a central tool to decipher complex processes of living cells. Giant unilamellar vesicles (GUVs) are of particular interest as they are cell-like in size, observable with optical microscopy and ... -
SNARE-mediated membrane fusion on pore-spanning membranes – several fusion pathways analyzed by single-vesicle content release
(2021-01-08)The fusion of neurotransmitter filled vesicles with the presynaptic membrane is the key step in the neuronal signaling cascade and is mediated by soluble N-ethylmaleimide-sensitive factor attachment receptor proteins ... -
Preparation and investigation of an in vitro model system for the GABAA receptor organisation machinery of inhibitory post synapses
(2020-07-22)The synaptic communication between neuronal cells is based on the release and uptake of neurotransmitters. In the mammalian brain inhibitory signal transduction relies on the neurotransmitter γ-amino butyric acid (GABA) ... -
Lipid dependent interactions of biomimetic membrane models with the ENTH domain of epsin
(2020-02-05)Membrane remodeling processes during endocytosis are highly regulated by the protein-protein and protein-membrane interactions. In clathrin-mediated endocyto-sis (CME) binding of the protein epsin to its receptor lipid ... -
Self-Organization and Mechanics of Minimal Actin Cortices attached to artificial Bilayers
(2018-10-23)One of the most versatile used protein structure in nature are actin filaments. They form contractile structures, like muscle fibrils, as well as complex network structures like the cellular cortex. The actin cortex is ... -
Pore-spanning membranes – a versatile tool to analyze SNARE-mediated single vesicle fusion
(2018-06-27)Signal transmission in neurons and hormone secretion in neuroendocrine cells such as chromaffin cells are fundamental biological processes including SNARE- (soluble N-ethylmaleimide-sensitive-factor attachment receptor) ... -
Pore-spanning lipid membranes as a tool to study membrane permeabilization by antimicrobial peptides
(2017-09-06)Antimicrobial peptides (AMPs) have a high therapeutic potential in combating microbial diseases. Their modes of action are still not well understood, and screening methods to identify and improve AMPs are needed. This work ... -
Structure and dynamics of artificial lipid membranes containing the glycosphingolipid Gb3
(2015-09-07)The lateral structure of the plasma membrane of mammalian cells is governed by the underlying actin cytoskeleton and the complex composition of the membrane. Using phase-separated membranes of the raft-like mixture ... -
A scanning ion conductance microscopy assay to investigate interactions between cell penetrating peptides and pore-suspending membranes
(2014-10-10)Scanning ion conductance microscopy (SICM) provides a technique for the investigation of surface topography as well as the local ion conductance of a surface without any mechanical contact between probe and sample. This ...