Browsing GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften by Referee "Jahn, Reinhard Prof. Dr."
Now showing items 1-20 of 49
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Fluorescence Correlates of Single Vesicles to Analyze the Behavior of Membrane-manipulating Proteins
(2025-01-24)Soluble NSF-attachment protein receptors (SNAREs) are integral to eukaryotic membrane fusion, providing the necessary energy to surmount the repulsive forces between two opposing membranes. This is achieved through the ... -
Transport mechanisms of the putative vesicular nucleotide transporter VNUT
(2024-12-13)The vesicular nucleotide transporter VNUT (SLC17A9) is thought to refill recycled synaptic vesicles and other secretory vesicles with adenosine triphosphate (ATP) for chemical signal transmission. ATP translocation by VNUT ... -
Characterization of Ca2+-triggered (de)ubiquitination events in depolarized synaptosomes by mass spectrometry
(2024-08-15)Communication between neurons takes place at synapses, where the transmission of information from the presynaptic neuron to the postsynaptic cell involves the Ca2+-regulated fusion of synaptic vesicles (SVs) with the ... -
A Quantitative Mass Spectrometric Analysis of Signalling Proteins in Human Sperm Cells
(2024-05-17)Fertilization of an oocyte by a sperm cell is a complex multi-stage process. First, a sperm cell must locate and reach the oocyte; second, it must undergo capacitation, the acrosome reaction, and hyperactivation to penetrate ... -
Biochemical reconstitution of human autophagy initiation
(2023-05-04)Autophagy is a tightly regulated process that eukaryotic cells use as a major survival mechanism to reallocate nutrients to essential processes in adverse conditions such as nutrient or energy deprivation. Autophagy is ... -
The Dynamic Organization of Proteins in the Synaptic Bouton
(2022-09-12)Protein availability is crucial for maintaining cellular processes. In synapses, the identities and functions of proteins involved in neurotransmission are well known. Their spatial distribution, copy numbers, and lifetimes ... -
Kinetic analysis of neuronal SNARE protein interactions
(2022-08-08)When an action potential arrives at the axon terminal, it causes a local and transient increase in the Ca2+ concentration that starts a cascade of molecular interactions eventually leading to the fusion of synaptic vesicles ... -
Targeting ion sensors to synaptic vesicles using a Nanobody against luminal Synaptotagmin 1
(2022-02-24)The synapse is a specialized nanostructure that connects two neurons where the ionic composition in each of its nano compartments is the driving process for synaptic transmission. Synaptic transmission relies on the ... -
Mitochondrial adaptation at the neuronal presynapse
(2021-11-22)Synaptic transmission poses a major energy consuming process in the brain, but how neurons maintain a constant energy supply during extended synaptic activity and how presynaptic mitochondria contribute to the energy ... -
Post-Translational Protein Modifications involved in Exo- and Endocytosis of Synaptic Vesicles
(2021-10-26)Neurotransmitter release is a key step that enables information flow between the pre- and post-synapse. However, regulation of the neurotransmitter release remains an intricate and widely unexplored matter despite recent ... -
Effects of arginine derivatives and oligopeptides on the physical properties of model membranes
(2021-02-16)Cell-penetrating peptides (CPPs) are often rich in arginine. They are able to cross cell membranes and carry other molecules into cells without being cytotoxic. The mechanisms by which they accomplish this are still ... -
Preparation and investigation of an in vitro model system for the GABAA receptor organisation machinery of inhibitory post synapses
(2020-07-22)The synaptic communication between neuronal cells is based on the release and uptake of neurotransmitters. In the mammalian brain inhibitory signal transduction relies on the neurotransmitter γ-amino butyric acid (GABA) ... -
Molecular mechanisms of synaptic vesicle recycling with a focus on Endophilin A and Rabconnectin-3a
(2019-11-01)Synaptic vesicle (SV) recycling at the synapse is fundamental to neurotransmission and it sustains unimpaired neuronal communication. It is a complex cell biological process, where the molecular steps are finely coordinated ... -
Pore-spanning membranes – a versatile tool to analyze SNARE-mediated single vesicle fusion
(2018-06-27)Signal transmission in neurons and hormone secretion in neuroendocrine cells such as chromaffin cells are fundamental biological processes including SNARE- (soluble N-ethylmaleimide-sensitive-factor attachment receptor) ... -
Study of SNARE-mediated membrane fusion with a novel single vesicle fusion assay
(2018-06-12)Membrane fusion in the secretory pathway of eukaryotic cells, including exocytosis of synaptic vesicles, is catalysed by SNARE proteins. In case of the synaptic vesicle fusion, two of the members of this protein family ... -
Biochemical characterization of presynaptic membrane protein complexes
(2017-09-13)At chemical synapses, synaptic vesicles, membrane-enclosed entities filled with neurotransmitters, undergo exocytosis at restricted areas of the presynaptic membrane called active zones (AZ). The core AZ proteins form a ... -
Long-Term Temporal Dynamics of Synaptic Vesicles
(2017-09-11)Neurotransmission requires the release of neurotransmitters from synaptic vesicles. This occurs via fusion of the vesicle to the pre-synaptic membrane upon stimulation. However, not all synaptic vesicles are equally ... -
Pore-spanning lipid membranes as a tool to study membrane permeabilization by antimicrobial peptides
(2017-09-06)Antimicrobial peptides (AMPs) have a high therapeutic potential in combating microbial diseases. Their modes of action are still not well understood, and screening methods to identify and improve AMPs are needed. This work ... -
A comprehensive characterization of brain and retina synaptic vesicle proteome by quantitative mass spectrometry.
(2017-06-07)Synaptic vesicles (SVs) are essential organelles for the transfer of information between a pre-synaptic nerve terminal and a post-synaptic target. SVs release their neurotransmitter content into the synaptic cleft in a ... -
Functional characterization of npcRNAs - Intercellular trafficking of generegulatory components via exosomes
(2017-04-11)Exosomes are small extracellular vesicles (EVs) of endocytic origin and get released by a multitude of cell types. These vesicles have been found in a variety of body fluids and more recently an important role in exchanging ...