Browsing GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften by Referee "Jahn, Reinhard Prof. Dr."
Now showing items 21-40 of 45
-
Molecular profiling of presynaptic docking sites
(2011-10-20)In presynaptic nerve endings, exocytosis of synaptic vesicles is restricted to specialized areas of the plasma membrane, called active zones, that are distinguished by electron-dense material. Here synaptic vesicles ... -
Myelin Membrane Growth and Organization in a Cellular Model System (EN)
(2011-01-19)This thesis addresses the two fundamental questions of myelin biogenesis: how the myelin membrane is extended and how it acquires its specific lateral organization. Myelin is a multilayered ... -
Non-Canonical Amino Acids as Minimal Tags for Investigating Protein Organization and Turnover
(2016-01-08)The burgeoning field of genetic code expansion provides new tools for specifically labeling proteins for a variety of applications. Clickable non-canonical amino acids (ncAA) have been refined for almost-quantitative and ... -
Phosphorylation of Synaptotagmin 4 captures transiting dense core vesicles at active synapses
(2017-03-03)Synaptic modulation requires fast recruitment of neuronal dense core vesicles (DCVs) containing various neuropeptides and neurotrophins at nerve terminals. DCVs undergo long-range trafficking in axons to deliver cargoes ... -
Pore-spanning lipid membranes as a tool to study membrane permeabilization by antimicrobial peptides
(2017-09-06)Antimicrobial peptides (AMPs) have a high therapeutic potential in combating microbial diseases. Their modes of action are still not well understood, and screening methods to identify and improve AMPs are needed. This work ... -
Pore-spanning membranes – a versatile tool to analyze SNARE-mediated single vesicle fusion
(2018-06-27)Signal transmission in neurons and hormone secretion in neuroendocrine cells such as chromaffin cells are fundamental biological processes including SNARE- (soluble N-ethylmaleimide-sensitive-factor attachment receptor) ... -
Post-Translational Protein Modifications involved in Exo- and Endocytosis of Synaptic Vesicles
(2021-10-26)Neurotransmitter release is a key step that enables information flow between the pre- and post-synapse. However, regulation of the neurotransmitter release remains an intricate and widely unexplored matter despite recent ... -
Preparation and investigation of an in vitro model system for the GABAA receptor organisation machinery of inhibitory post synapses
(2020-07-22)The synaptic communication between neuronal cells is based on the release and uptake of neurotransmitters. In the mammalian brain inhibitory signal transduction relies on the neurotransmitter γ-amino butyric acid (GABA) ... -
Quantitative phosphoproteomics for studying B-cell receptor signaling in Burkitt’s lymphoma
(2017-02-16)Protein phosphorylation plays an important role in transmitting cellular signals. Phosphorylation has been mostly characterized by hypothesis-driven and low-throughput approaches in the past. However, novel phosphorylation ... -
Reconstituted SNARE-mediated fusion: towards a mechanistic understanding
(2012-03-20)A novel and well-characterized model system has been developed and used in order to extract mechanistic insights on the SNARE-mediated fusion process. The reconstitution of SNAREs onto large ... -
Regulation of recycling endosomal membrane traffic by a γ-BAR/ kinesin KIF5 complex
(2008-01-15)Intracellular trafficking of membrane proteins and lipids between different compartments is a fundamental process underlying a variety of cell physiological functions of eukaryotic cells. ... -
Role of DLG-MAGUKs in surface NMDAR localization and its patho-physiological functions
(2014-10-16)The post-synaptic side of an excitatory synapse is characterized by an electron-dense, highly dynamic architecture of proteins, called the post-synaptic density or PSD. PSDs comprises of a diverse group of proteins, from ... -
Role of Munc13 Isoforms in Regulating Large Dense Core Vesicle Exocytosis in Chromaffin Cells
(2015-11-03)The process of vesicular exocytosis is a fast, tightly regulated and Ca2+-triggered event mediated by SNARE complex formation. Although it is known that factors like Munc18, Munc13 and CAPS are important in setting up ... -
Spatial-temporal actin dynamics during synaptic plasticity of single dendritic spine investigated by two- photon fluorescence correlation spectroscopy
(2013-12-19)In the content of my thesis, the construction and calibration of a setup that combined conventional wide field fluorescence microscopy (FM) and two-photon fluorescence correlation spectroscopy (2P-FCS) was first described. ... -
Studies towards understanding the regulation and function of <i>bällchen</i> in different stem cell systems of <i>Drosophila melanogaster</i>
(2009-01-23)Bällchen (BALL) is a conserved Serine/Threonine kinase of Drosophila melanogaster suggested to participate in stem cell function. Here, I report the pattern of the ball transcripts and the ... -
Study of SNARE-mediated membrane fusion with a novel single vesicle fusion assay
(2018-06-12)Membrane fusion in the secretory pathway of eukaryotic cells, including exocytosis of synaptic vesicles, is catalysed by SNARE proteins. In case of the synaptic vesicle fusion, two of the members of this protein family ... -
Synthesis and Analysis of Modified SNARE Proteins with Respect to Assembly and Disassembly of the SNARE Complex
(2017-01-12)In this thesis, the first aim was to synthesise an inhibitor for the alpha-SNAP/NSF-mediated disassembly reaction of the SNARE complex. Based on identified interaction sites between alpha-SNAP and Synaptobrevin-2, suitable ... -
Targeting ion sensors to synaptic vesicles using a Nanobody against luminal Synaptotagmin 1
(2022-02-24)The synapse is a specialized nanostructure that connects two neurons where the ionic composition in each of its nano compartments is the driving process for synaptic transmission. Synaptic transmission relies on the ... -
The Dynamic Organization of Proteins in the Synaptic Bouton
(2022-09-12)Protein availability is crucial for maintaining cellular processes. In synapses, the identities and functions of proteins involved in neurotransmission are well known. Their spatial distribution, copy numbers, and lifetimes ... -
The identification and characterization of Mio10 and MINOS1 as novel regulators of mitochondrial inner membrane organization
(2012-06-07)Various mitochondrial inner membrane proteins contribute to the structure of cristae membranes and to the overall dynamic morphology of the inner membrane. These proteins include the F1FOATPase dimers, which contribute to ...